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lipid-based vivo transfection reagent kit  (Altogen Labs)

 
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    Altogen Labs lipid-based vivo transfection reagent kit
    Regulation of BNIP3 controls the intracellular survival of Mtb in vivo . A C57BL/6 WT mice were intraperitoneally injected with siControl or siBINP3 (1.8 mg/kg) using the LIPID-based in vivo <t>transfection</t> reagent (6 mice per group). After 2 days, mice were intratracheally infected with Mtb (1 × 10 6 CFU), and then injected with siRNA (0.6 mg/kg) for 15 consecutive days, once every 3 days. B The levels of BNIP3, LC3, HIF1α, and β-actin were evaluated using western blotting. C Bacterial burden was measured in the lungs 15 days after infection ( n = 6 mice per group). D, E Levels of MCP-1 and TNF-α in the sera of Mtb-infected mice were analyzed by ELISA ( n = 6 mice per group). The data are presented as means ± SD of at least three independent experiments; * p < 0.05, *** p < 0.001
    Lipid Based Vivo Transfection Reagent Kit, supplied by Altogen Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lipid-based+vivo+transfection+reagent+kit/lipid+based+vivo+transfection+reagent+kit/pmc10470153-310-24-28
    Average 90 stars, based on 1 article reviews
    lipid-based vivo transfection reagent kit - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Impaired mitophagy induces antimicrobial responses in macrophages infected with Mycobacterium tuberculosis"

    Article Title: Impaired mitophagy induces antimicrobial responses in macrophages infected with Mycobacterium tuberculosis

    Journal: Cell & Bioscience

    doi: 10.1186/s13578-023-01107-2

    Regulation of BNIP3 controls the intracellular survival of Mtb in vivo . A C57BL/6 WT mice were intraperitoneally injected with siControl or siBINP3 (1.8 mg/kg) using the LIPID-based in vivo transfection reagent (6 mice per group). After 2 days, mice were intratracheally infected with Mtb (1 × 10 6 CFU), and then injected with siRNA (0.6 mg/kg) for 15 consecutive days, once every 3 days. B The levels of BNIP3, LC3, HIF1α, and β-actin were evaluated using western blotting. C Bacterial burden was measured in the lungs 15 days after infection ( n = 6 mice per group). D, E Levels of MCP-1 and TNF-α in the sera of Mtb-infected mice were analyzed by ELISA ( n = 6 mice per group). The data are presented as means ± SD of at least three independent experiments; * p < 0.05, *** p < 0.001
    Figure Legend Snippet: Regulation of BNIP3 controls the intracellular survival of Mtb in vivo . A C57BL/6 WT mice were intraperitoneally injected with siControl or siBINP3 (1.8 mg/kg) using the LIPID-based in vivo transfection reagent (6 mice per group). After 2 days, mice were intratracheally infected with Mtb (1 × 10 6 CFU), and then injected with siRNA (0.6 mg/kg) for 15 consecutive days, once every 3 days. B The levels of BNIP3, LC3, HIF1α, and β-actin were evaluated using western blotting. C Bacterial burden was measured in the lungs 15 days after infection ( n = 6 mice per group). D, E Levels of MCP-1 and TNF-α in the sera of Mtb-infected mice were analyzed by ELISA ( n = 6 mice per group). The data are presented as means ± SD of at least three independent experiments; * p < 0.05, *** p < 0.001

    Techniques Used: In Vivo, Injection, Transfection, Infection, Western Blot, Enzyme-linked Immunosorbent Assay

    Related Articles

    In Vivo:

    Article Title: Impaired mitophagy induces antimicrobial responses in macrophages infected with Mycobacterium tuberculosis
    Article Snippet: .. Delivery of siRNA in vivo was intraperitoneally injected with negative control siRNA or mouse BNIP3 siRNA (Bioneer) following the instructions of the LIPID-based in vivo transfection reagent kit (Altogen Biosystems). ..

    Injection:

    Article Title: Impaired mitophagy induces antimicrobial responses in macrophages infected with Mycobacterium tuberculosis
    Article Snippet: .. Delivery of siRNA in vivo was intraperitoneally injected with negative control siRNA or mouse BNIP3 siRNA (Bioneer) following the instructions of the LIPID-based in vivo transfection reagent kit (Altogen Biosystems). ..

    Negative Control:

    Article Title: Impaired mitophagy induces antimicrobial responses in macrophages infected with Mycobacterium tuberculosis
    Article Snippet: .. Delivery of siRNA in vivo was intraperitoneally injected with negative control siRNA or mouse BNIP3 siRNA (Bioneer) following the instructions of the LIPID-based in vivo transfection reagent kit (Altogen Biosystems). ..

    Transfection:

    Article Title: Impaired mitophagy induces antimicrobial responses in macrophages infected with Mycobacterium tuberculosis
    Article Snippet: .. Delivery of siRNA in vivo was intraperitoneally injected with negative control siRNA or mouse BNIP3 siRNA (Bioneer) following the instructions of the LIPID-based in vivo transfection reagent kit (Altogen Biosystems). ..



    Similar Products

    lipid-based vivo transfection reagent kit  (Altogen Labs)
    90
    Altogen Labs lipid-based vivo transfection reagent kit
    Regulation of BNIP3 controls the intracellular survival of Mtb in vivo . A C57BL/6 WT mice were intraperitoneally injected with siControl or siBINP3 (1.8 mg/kg) using the LIPID-based in vivo <t>transfection</t> reagent (6 mice per group). After 2 days, mice were intratracheally infected with Mtb (1 × 10 6 CFU), and then injected with siRNA (0.6 mg/kg) for 15 consecutive days, once every 3 days. B The levels of BNIP3, LC3, HIF1α, and β-actin were evaluated using western blotting. C Bacterial burden was measured in the lungs 15 days after infection ( n = 6 mice per group). D, E Levels of MCP-1 and TNF-α in the sera of Mtb-infected mice were analyzed by ELISA ( n = 6 mice per group). The data are presented as means ± SD of at least three independent experiments; * p < 0.05, *** p < 0.001
    Lipid Based Vivo Transfection Reagent Kit, supplied by Altogen Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lipid-based+vivo+transfection+reagent+kit/lipid+based+vivo+transfection+reagent+kit/pmc10470153-310-24-28
    Average 90 stars, based on 1 article reviews
    lipid-based vivo transfection reagent kit - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Regulation of BNIP3 controls the intracellular survival of Mtb in vivo . A C57BL/6 WT mice were intraperitoneally injected with siControl or siBINP3 (1.8 mg/kg) using the LIPID-based in vivo transfection reagent (6 mice per group). After 2 days, mice were intratracheally infected with Mtb (1 × 10 6 CFU), and then injected with siRNA (0.6 mg/kg) for 15 consecutive days, once every 3 days. B The levels of BNIP3, LC3, HIF1α, and β-actin were evaluated using western blotting. C Bacterial burden was measured in the lungs 15 days after infection ( n = 6 mice per group). D, E Levels of MCP-1 and TNF-α in the sera of Mtb-infected mice were analyzed by ELISA ( n = 6 mice per group). The data are presented as means ± SD of at least three independent experiments; * p < 0.05, *** p < 0.001

    Journal: Cell & Bioscience

    Article Title: Impaired mitophagy induces antimicrobial responses in macrophages infected with Mycobacterium tuberculosis

    doi: 10.1186/s13578-023-01107-2

    Figure Lengend Snippet: Regulation of BNIP3 controls the intracellular survival of Mtb in vivo . A C57BL/6 WT mice were intraperitoneally injected with siControl or siBINP3 (1.8 mg/kg) using the LIPID-based in vivo transfection reagent (6 mice per group). After 2 days, mice were intratracheally infected with Mtb (1 × 10 6 CFU), and then injected with siRNA (0.6 mg/kg) for 15 consecutive days, once every 3 days. B The levels of BNIP3, LC3, HIF1α, and β-actin were evaluated using western blotting. C Bacterial burden was measured in the lungs 15 days after infection ( n = 6 mice per group). D, E Levels of MCP-1 and TNF-α in the sera of Mtb-infected mice were analyzed by ELISA ( n = 6 mice per group). The data are presented as means ± SD of at least three independent experiments; * p < 0.05, *** p < 0.001

    Article Snippet: Delivery of siRNA in vivo was intraperitoneally injected with negative control siRNA or mouse BNIP3 siRNA (Bioneer) following the instructions of the LIPID-based in vivo transfection reagent kit (Altogen Biosystems).

    Techniques: In Vivo, Injection, Transfection, Infection, Western Blot, Enzyme-linked Immunosorbent Assay